commsp

Title Body Location
Other species

Some Dinobryaceae occures,
minor Diatoms (Navicula)

Shore of Abiquiu Lake (desert environment), floating bloom on the surface of the water [Abiquiu 1]
Overview

DNA+
Pcr+
Cloning required
Plated to agar F/20 (ppt 15), F/20 (ppt 60), F/100 (ppt 60), BG11 (ppt 0), and to liquid F/20 (ppt 15).

Baffin bay, Laguna Madre (BB-D16)
Overview

DNA+
Pcr+
Cloning required
Plated to agar F/20 (ppt 15), F/20 (ppt 60), F/100 (ppt 60), BG11 (ppt 0), and to liquid F/20 (ppt 15).

Isolate from Vicinity of Corpus Christi, Laguna Madre, petri dish (Salt Marsh 1.4). Taking biomass for DNA extraction.
Overview

DNA extraction required
Pcr required
Cloning required
Plated to agar F/20 (ppt 15), F/20 (ppt 60), F/100 (ppt 60), BG11 (ppt 0), and to liquid F/20 (ppt 15).

Subcultured material from Petri dish (SMPZ1). Material have been collected in vicinity of Corpus Christi, Laguna Madre.
Pcr with 16S primers did not

Pcr with 16S primers did not work

Subcultured material from Petri dish (BA2 NQ4). Material have been collected in vicinity of Corpus Christi, Baffin Bay, Laguna Madre. DNA
Pcr with 16S primers did not

Pcr with 16S primers did not work, probably because of low yield of DNA and purity of material.

Subcultured material from glass Petri dish labeled with BA2.7 SHELL3. Material have been collected in vicinity of Corpus Christi, Laguna Madre, Baffin Bay.
Petri

Petri dish labeled [EB2_2ul_1_p] was derived from current tube

Test tube in the lab [EB2_2ul], mix of cyanobacteria and algae (Desmodesmus sp. Ceratium sp.). Derived from following sample http://kpabg.ru/cyanopro/?q=node/96612, Elephant Butte Lake
Petri

Petri dish labeled [EB2_3ul_1_p] was derived from current tube

Tube [EB2_3ul] with the subculture from following record http://kpabg.ru/cyanopro/?q=node/96612, Elephant Butte Lake
Phylogeny

Separate clade from Stanieria cyanosphaera sensu stricto and 'Guido'

SAG 33.87
Plate

Petri dish labeled [EB3_1uL_1_p] was derived from current tube

Tube labeled [EB3_1uL], Elephant Butte Lake

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